Novel aspects of the Z and R3 antigens of streptococcus agalactiae revealed by immunological testing.

Simple item page
dc.contributor.authorMaeland, Johan A.
dc.contributor.authorRadtke, Andreas
dc.contributor.authorLyng, Randi V.
dc.contributor.authorMavenyengwa, Rooyen T.
dc.date.accessioned2013-08-01T18:09:54Z
dc.date.available2013-08-01T18:09:54Z
dc.date.issued2013
dc.description.abstractGroup B streptococci (GBS) are important human and bovine pathogens which can be classified by a variety of phenotype- and gene-based techniques. The capsular polysaccharide and strain-variable, surface-anchored proteins are particularly important phenotypic markers. In an earlier study, a previously unrecognized protein antigen called Z was described. It was expressed by 27.2% of GBS strains from Zimbabwe, usually in combination with R3 protein expression. In this study, a putative Z-specific antiserum actually contained antibodies against two different antigens named Z1 and Z2; Z1 was>250 kDa in molecular mass. Z1, Z2, and R3 generated multiple stained bands on Western blots and showed similar chromatographic characteristics with respect to molecular mass, aggregate formation, and charge. Of 28 reference and prototype GBS strains examined, 8/28 (28.5%) isolates expressed one, two, or all three of the Z1, Z2, and R3 antigens; 4/28 expressed all three antigens; 2/28 expressed Z2 and R3; 1/28 expressed Z1 only; and 1/28 expressed R3 only. Twenty (71.5%) of the 28 isolates expressed none of the three antigens. Expression of one or more of these antigens was shown by isolates of the capsular polysaccharide types Ia, Ib, V, and IX and NT strains and occurred in combination with expression of various other strain-variable and surface-localized protein antigens. When used as serosubtype markers, Z1, Z2, and R3 affected existing GBS serotype designations for some of the isolates. For instance, the R3 reference strain Prague 10/84 (ATCC 49447) changed serotype markers from V/R3 to V/R3, Z1, and Z2. Other isolates may change correspondingly, implying consequences for GBS serotyping and research.en_US
dc.identifier.citationMaeland, J. A., Radtke, A., Lyng, R. V., & Mavenyengwa, R. T. (2013). Novel aspects of the Z and R3 antigens of streptococcus agalactiae revealed by immunological testing. Clinical and Vaccine Immunology, 20(4), 607-612.en_US
dc.identifier.issn1556-679X
dc.identifier.urihttp://hdl.handle.net/10628/386
dc.language.isoenen_US
dc.publisherASM: American Society for Microbiologyen_US
dc.subjectGroup B streptococci (GBS)en_US
dc.subjectGBS strainsen_US
dc.titleNovel aspects of the Z and R3 antigens of streptococcus agalactiae revealed by immunological testing.en_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Mavenyengwa. Novel Aspects of the Z and R3 antigens of stretococcus.pdf
Size:
251.36 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Health Sciences